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Overview of the CAM schedule from EDD0 to EDD17. In general, chick embryo incubation is marked as embryonic day of development 0 (EDD0). The puncturing day (EDD3) allows the translocation of the natural air sac to the top of the egg. Grafting of 2 × 106 SCC-25 cells (EDD6) enables the generation of a solid visible tumor at 4 days post-grafting (EDD10). The topical treatment is applied on EDD10, and the tumor mass is monitored until EDD17, the last day of incubation (harvesting).
