Fig. 4.

NETs and neutrophil proteins in the sputum of patients receiving rhDNase. Sputum could be collected from four of five patients who received rhDNase treatment. Widefield microscopy of neutrophil elastase (magenta, antielastase ab) and DNA (cyan, DAPI) in sputum samples from treated patients (TP 1–4). Insets show stitched overview image from 6 × 6 images of the samples. Quantification of single cell–level NETs (percent of cells, average NET size, and number of cells) of each overview is shown in the top corner of representative images. The scale bars are the same for each image and corresponds to 50 μm (high magnification) or 500 μm (overview inset), respectively. Day of. sputum sampling is indicated in the bottom left corner. For some images, there were too few cells (<100) identified for reliable quantification, and thus they were excluded from the analysis. The images shown in this figure are part of the NETs quantification in Figure 5, A and B. DAPI, 4,6 diamidino-2-phenylindole; NETs, neutrophil extracellular traps; rhDNase, recombinant human DNase I.