| Strain, strain background (Ambystoma mexicanum, d/d strain) |
Axolotl, d/d strain |
Tanaka lab axolotl colony |
- |
Axolotl stock maintained in Tanaka lab, Vienna, Austria. |
| Genetic reagent (Ambystoma mexicanum, AxFUCCI transgenic) |
Transgenic AxFUCCI axolotl |
This paper |
- |
Transgenic stock generated in d/d genetic background in Tanaka lab, Vienna, Austria. |
| Gene (Ambystoma mexicanum) |
Cdt1 |
Axolotl genome release v6.0-DD Schloissnig, 2021
|
CDT1|AMEX60DD201052583.1 |
Axolotl genome release v6.0-DD available at: https://www.axolotl-omics.org/assemblies. |
| Gene (Ambystoma mexicanum) |
Gmnn |
Axolotl genome release v6.0-DD Schloissnig, 2021
|
GMNN|AMEX60DD301038574.1 |
Axolotl genome release v6.0-DD available at: https://www.axolotl-omics.org/assemblies. |
| Cell line (AL1, Ambystoma mexicanum) |
AL1 cells |
Roy et al., 2000
|
- |
AL1 cells were kindly provided by Dr David Gardiner (UC Irvine). AL1 cells are an immortalized mesenchymal axolotl line originally derived from a limb blastema. |
| Recombinant DNA reagent |
Addgene plasmid mVenus N1 |
Addgene |
RRID:Addgene_27793
|
To amplify coding sequence for mVenus fluorescent protein by PCR. |
| Recombinant DNA reagent |
pAxFUCCI |
This paper |
pAxFUCCI |
Plasmid encoding AxFUCCI. Used to generate AxFUCCI transgenic axolotls by I-SceI-mediated transgenesis. |
| Sequence-based reagent |
axCdt1-fwd |
This paper |
PCR primer |
TCATGCGGCCGCATGGCCCAGCTCCGGATGA.Forward primer to amplify axCdt1[aa1-128] from axolotl embryonic cDNA. Bold indicates a NotI restriction site. |
| Sequence-based reagent |
axCdt1-rev |
This paper |
PCR primer |
TCATGCTAGCGAATTCTCCGCTTCCTGCTGCGCTTCCTGCGCTTCCCAGGGATGATGGG GTTAATGGCTReverse primer to amplify axCdt1[aa1-128] from axolotl embryonic cDNA. Bold indicates a NheI restriction site. Underlined sequence encodes GS-rich linker. |
| Sequence-based reagent |
Venus-fwd |
This paper |
PCR primer |
TCATGCTAGCATGGTGAGCAAGGGCGAGGForward primer to amplify mVenus sequence from addgene plasmid #27793. Bold indicates a NheI restriction site. |
| Sequence-based reagent |
Venus-rev |
This paper |
PCR primer |
TCATACCGGTCTTGTACAGCTCGTCCATGCCReverse primer to amplify mVenus sequence from addgene plasmid #27793. Bold indicates a AgeI restriction site. |
| Sequence-based reagent |
axGmnn-fwd |
This paper |
PCR primer |
TCATTCCGGAGGAGGAGGAGGAAGCGGAGGAGGAGGAAGCATGAATGCTAAGAAAG CAGCGACAATForward primer to amplify axGmnn[aa1-93] from axolotl embryonic cDNA. Bold indicates a BspEI restriction site. Underlined sequence encodes GS-rich linker. |
| Sequence-based reagent |
axGmnn-rev |
This paper |
PCR primer |
TCATATCGATGCTGTAAGCTTCTTGGGACA CCCReverse primer to amplify axGmnn[aa1-93] from axolotl embryonic cDNA. Bold indicates a ClaI restriction site. |
| Antibody |
Mouse monoclonal antibody to NeuN |
Millipore |
MAB377RRID:AB_2298772
|
1:500 |
| Antibody |
Rabbit polyclonal antibody to Sox2 |
Fei et al., 2016
|
- |
1:1000 |
| Commercial assay or kit |
Click-iT 647 EdU detection kit for Imaging |
Thermo Fisher Scientific |
C10340 |
To label cells that have transited through S-phase. |
| Chemical compound, drug |
Easyindex |
LifeCanvas Technologies |
- |
Refractive index matching solution for light sheet imaging (RI 1.46). |
| Software, algorithm |
Trackmate plugin for Fiji |
Tinevez et al., 2017
|
- |
To track AL1 cells for the purposes of quantifying AxFUCCI fluorescence. |
