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. 2021 May 5;10:e67784. doi: 10.7554/eLife.67784

Figure 4. Phenotypic classification based on glucosinolate (GSL) content.

(A) Using the GSL accumulation, each accession was classified to one of seven aliphatic short-chained GSL chemotypes based on the enzyme functions as follows: MAM2, AOP null: classified as 3MSO dominant, colored in yellow. MAM1, AOP null: classified as 4MSO dominant, colored in pink. MAM2, AOP3: classified as 3OHP dominant, colored in green. MAM1, AOP3: classified as 4OHB dominant, colored in light blue. MAM2, AOP2: classified as Allyl dominant, colored in blue. MAM1, AOP2, GS-OH non-functional: classified as 3-Butenyl dominant, colored in black. MAM1, AOP2, GS-OH functional: classified as 2-OH-3-Butenyl dominant, colored in red. The accessions were plotted on a map based on their collection sites and colored based on their dominant chemotype. (B) The coloring scheme with functional GSL enzymes in the aliphatic GSL pathway is shown with the percentage of accessions in each chemotypes (out of the total 797 accessions) shown in each box.

Figure 4—source data 1. Environmental conditions differentially associate with MAM status in the north versus the south.
Linear model for MAM status (carbon side chain length) was conducted with the indicated environmental parameters, for the northern and southern collection, separately (for more details, see Methods). The tables show p values for each term from the linear model. For the interaction with geography, the linear model was run using the total dataset, and the geography parameter (north or south) was added to the model.

Figure 4.

Figure 4—figure supplement 1. Phenotypic classification based on the dominant MAM enzyme.

Figure 4—figure supplement 1.

Accessions were classified based on the side chain length of the aliphatic short-chained glucosinolates (GSLs). Accessions with a majority of GSLs containing three carbons in their side chains are classified as MAM2 dominant and colored in blue. Accessions with the majority of aliphatic short-chained GSLs containing four carbons in their side chains are classified as MAM1 dominant and colored in red. The accessions were plotted on a map based on their original collection sites.
Figure 4—figure supplement 2. Phenotypic classification based on the dominant AOP enzymes.

Figure 4—figure supplement 2.

Relative amounts of alkenyl glucosinolates (GSLs), alkyl GSLs and methylsulfinyl (MSO) GSLs were calculated in respect to the total short-chained aliphatic GSLs as described in the Methods section. Accessions with high amounts of alkenyl GSLs were classified as AOP2 dominant and colored in pink. Accessions with high amounts of alkyl GSLs were classified as AOP3 dominant and colored in orange. Accessions with high amounts of MSO GSLs were classified as AOP null and colored in green. The accessions were plotted on a map based on their original collection sites.
Figure 4—figure supplement 3. Phenotypic classification based on GS-OH enzyme activity.

Figure 4—figure supplement 3.

The ratio between 2-OH-3-Butenyl to 3-Butenyl glucosinolate (GSL) was calculated only for MAM1-dominant accessions (accessions with GSLs containing four carbons in their side chain). Accessions with high amounts of 2-OH-3-Butenyl were classified as GS-OH functional and colored in black. Accessions with mostly 3-Butenyl were classified as GS-OH non-functional and colored in brown. The accessions were plotted on a map based on their original collection sites.
Figure 4—figure supplement 4. Geographic partitioning of the collection.

Figure 4—figure supplement 4.

(A) The accessions were divided to two collections using the following chain of mountains: the Pyrenees between Spain and France, the Alps between Italy and Germany, and the Carpathians in the Balkan. The accessions that are located north of these mountains are referred to as the northern accessions and colored in green. The accessions located south of these mountains are referred to as the southern accessions and colored in pink. (B) The percentage of each chemotype was independently calculated in the south and north. Butenyl: 3-Butenyl; OH-But: 2-OH-3-Butenyl.