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. 2021 May 20;8:644244. doi: 10.3389/fmed.2021.644244

Figure 4.

Figure 4

STAT2 signaling fails to restore Paneth cell viability but alters inflammation. (A) Representative images of small intestinal tissue cross sections of control, Casp8ΔIEC, Casp8ΔIECxStat1+/−Stat2−/− and Casp8ΔIECxStat1−/−Stat2−/− mice stained with H&E and PAS (scale bar: 100 μm). (B) Western blot analysis and normalization of ileal tissue with antibodies against STAT1 and Lysozyme. β-Actin was used as loading control. Densitometry analysis for quantification (n ≥ 2). Error bars indicate +SD. (C) Gene transcription analysis of Mlkl mRNA expression in the small intestine. Gapdh was used as housekeeping gene. Gene expression levels are shown as fold changes. Error bars indicate +/-SD. Statistical analyses: One-way ANOVA with Tukey's multiple comparisons test; NS p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.