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Opa1-mediated mitophagy increases the activity of cellular antioxidant enzymes. Control (nontransduced) and Ad-Opa1-transduced H9C2 cardiomyocytes were treated with 0.3 mM H2O2 for 12 h. (a–c) Colorimetric determination of GSH, SOD, and GPX activities. (d, e) Results of qPCR assays to analyze the transcriptional profiles of Nrf2 and HO-1. ∗p < 0.05 vs. control group, #p < 0.05 vs. H2O2+Ad-Ctrl group.
