Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 30;35(1):58–69. doi: 10.1093/humrep/dez253

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

PMC Copyright notice

LtfCre ^0/+ ; (Gt)R26Pik3ca ^*H1047R ; Arid1a ^fl/+ and LtfCre ^0/+ ; (Gt)R26Pik3ca ^*H1047R ; Arid1a ^fl/fl mice develop ovarian lesions, anastomosis and ascites following salpingectomy. (A) Diagram depicting mouse alleles used in this study. ARID1A, AT-rich interactive domain-containing protein 1A; PIK3CA, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha; mEGFP, mouse enhanced green fluorescent protein. (B) Schematic representation of uterotubal incision and salpingectomy surgery. (C) Timeline for experimental mouse model. (D) Survival of mice following surgery. Survival was based on vaginal bleeding and other endpoints (see Table II). Control mice were aged for 1 year (n = 9, black line). No difference in survival was observed between LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/+ (n = 8, blue line) and LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl (n = 9, purple line) mice following surgery (P = 0.7421, unpaired t test, two-tailed). (E) Age at surgery vs. age at death. No significant relationship was observed (P = 0.1151 for LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/+, blue line; P = 0.3584 for LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl, purple line Pearson’s). (F) Example of LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl mice with ascites following surgery. (G) Ovarian cysts are observed in animals 6 weeks post-salpingectomy on the ipsilateral ovary. Arrow indicates cyst. (H) Example of animal with hemorrhagic ‘chocolate cyst’ endometrioma on ovary ipsilateral to incision. Arrow indicates lesion. (I) Intestinal obstruction in mutant mice following surgery. White arrows denote intestines, black arrows denote omental metastasis. (J) Intestinal anastomosis following salpingectomy. Black arrows denote omental lesions. (K) Bright-field image and fluorescent green image (K′). EGFP fluorescence denotes Cre + endometrial epithelium on or near the ovary. Ov: ovary. (L) Histogram compiling observational data upon necropsy.