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. 2021 Jun 16;44(10):2003–2034. doi: 10.1007/s00449-021-02597-5

Fig. 6.

Fig. 6

The microbial adhesion to hydrocarbon test is initiated by washing fungal cells to remove contaminants, followed by a resuspension in buffer solution (PUM) until reaching a standardized optical density (DO). Thereafter, the hydrocarbon is added, and the mixture is stirred. After complete separation of phases, the organic phase is removed, and the optical density is measured again (a). The percentage value of microbial cell adhesion to hydrocarbons is obtained as in (b)