Figure 5.

The MOR mAb 3A5C7 regulates the proliferation of HCC by affecting p53. (A) Western blot analysis showed expression changes in MOR-, CD147- and p53/MAPK pathway-related molecules in two HepG2 and Huh7 cell lines exposed to 3A5C7. (B) Western blotting and real-time PCR were conducted to verify the knockdown of p53 (****P < 0.0001). (C) Flow cytometry analysis of the cell cycle in cells that were silenced in p53. (D) Tumor formation capability was rescued by downregulating p53 in HepG2 and Huh7 cells (*P < 0.05, **P < 0.01). (E) HepG2 and Huh7 cells transfected with si-NC or p53 siRNA were assessed for proliferation ability at 24, 48 and 72 hrs after 3A5C7 was applied by CCK8 analysis (*P < 0.05, ****P < 0.0001). Supplementary File Raw Blot Images (Figure 5A and 5B) for original blot images. Data were presented as the mean ± SEM. Student’s t-test and one-way ANOVA were used for statistical analysis.