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. 2021 May 15;13(5):4760–4770.

Figure 5.

Figure 5

Validation of the association between miR-133 and GDNF. A: Specific binding sites of miR-133 and GDNF; B: Dual-luciferase reporter assay results; C: qRT-PCR detection of the regulation of GDNF mRNA expression by miR-133; D, E: Western blot detection of the regulation of GDNF protein expression by miR-133; F: GDNF mRNA expression in cells treated with different concentrations of sevoflurane; G, H: GDNF protein expression in cells treated with different concentrations of sevoflurane. Compared with co-transfection of miR-133-NC + GDNF-WT group, @P<0.05; compared with normal group, ΔP<0.05; compared with NC group, *P<0.05. GDNF: glial cell-derived neurotrophic factor; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.