Figure 4.

RORA is a potential target of miR-18a-5p. (A) Potential downstream targets of miR-18a-5p. (B) Pathway enrichment analysis of target genes. (C) The expression profiles of miR-18a-5p and its target genes in response to miR-18a-5p mimic examined by RT-qPCR. (D) RORA expression in response to HAEC-exo or PBS treatment by RT-qPCR. (E) Potential binding sites of RORA to miR-18a-5p and its mutation sites. (F) The effect of miR-18a-5p mimic on RORA-WT/RORA-MT luciferase activity determined by dual-luciferase assays. (G) The binding ability of miR-18a-5p to RORA mRNA examined by RIP assay. The data are exhibited as mean ± SD. Significant differences between two groups were assessed using an unpaired t-test (D-G). The significant differences among the groups were compared by two-way (C, F and G) ANOVA, followed by Tukey’s test. *, #P < 0.05, &&P < 0.01. Data are representative of 3 independent experiments.