Figure 6.

HAEC-exo exacerbates the inflammatory responses and apoptosis in HEAC mice. (A) miR-18a-5p and RORA expression in the colonic tissues of mice by RT-qPCR. (B) Expression of MUC1 and p-NFκB in the colonic tissue of mice examined by immunohistochemistry. (C) The concentration of pro-inflammatory factors in the serum of mice assessed by ELISA assay. (D) Bacterial counts in colonic tissue of mice. (E) Pathological changes in colonic tissue examined by HE staining. There are 6 mice in each group, and the images shown are representative ones. The significant differences among the groups were compared by one-way (D and E) or two-way (A-C) ANOVA, followed by Tukey’s test. *, #, &P < 0.05.