Table 3.

Comparison of the QCI-I results for chosen primary tumor duplicates with reported data on gene variant pathogenicity

Gene	Variant	Tier	Pathogenicity	Effect	Allelic frequency (%)
					Sample BC4 FFPE	Sample BC5 FFPE
EGFR	c.2125G>A, p.E709K	2C	ü	missense	4,81	-
PIK3CA	c.3073A>G, p.T1025A	2C	ü	missense	-	11
KIT	c.1737T>C, p.D579D	4	possibly not	missense	4,17	-
					Sample BC6 FFPE	Sample BC7 FFPE
PIK3CA	c.1049A>G, p.D350G	2C	ü	missense	4,49	-
					Sample BC11 FFPE	Sample BC12 FFPE
EGFR	c.2508C>T, p.R836R	4	possibly not	synonymous	26	42
PDGFRA	c.661C>T, p.L221F	4	possibly not	missense	36	40