Figure 8.

Detection of the percentages of CD8⁺ T cells (Thy1.1⁺) that specifically recognize OVA_257-264 in the context of H-2K^b by Flow cytometry analysis. The B16-OVA tumor-bearing mice received adoptive transfer of OT-I spleen cells when LV-OVA, LV/bPEI25k-OVA, or OVA alone were injected into inguinal lymph nodes. The lymphocytes were stained with APC-labeled anti-mouse Thy1.1 antibody, PerCP-labeled anti-mouse CD8 antibody and MHC Dextramer H-2K^b/SIINFEKL conjugated with PE. A. Representative cytoflourograph. B. Cumulative data from three experiments. *P<0.05, **P<0.01.