Figure 2.

Co-culture of hepatocellular carcinoma cell lines with human primary monocytes. A and B. Flow cytometric detection results and statistical analysis of human primary CD14⁺ monocytes after co-culture with three hepatocellular carcinoma cell lines for 2 days. C and D. ELISA detection of PGE2 and TGF-β in culture medium after co-culture of three hepatocellular carcinoma cell lines with human primary CD14⁺ monocytes for 2 days. E and F. Flow cytometric results and statistical analysis of CD14⁺ monocytes after adding celecoxib to the co-culture system of SNU423 cells and primary human CD14⁺ monocytes. G and H. ELISA detection of PGE2 and TGF-β in culture medium after adding celecoxib to the co-culture system of SNU423 cells and primary human CD14⁺ monocytes. Values are the mean ± SEM of a minimum of 3 independent experiments.