Figure 5.
Verification and analysis of in vivo models. A and B. Results and statistical analysis of different treatment regimes in NPG mouse after tumor formation. C and D. Flow cytometry results and statistical analysis of M2 macrophages (CD163/CD206) derived from human CD14+ monocytes in tumor tissues given different treatment regimes. E. ELISA detection and statistical analysis of PGE2 in in tumor tissues given different treatment methods. F. ELISA detection and statistical analysis of TGF-β in in tumor tissues given different treatment methods. G and H. ELISA detection and statistical analysis of IFN-γ and Granzyme B in human CD8+ T cells after sorting from tumor tissues. I. Western results for members of the TGF-β pathway. J-N. Statistical analysis of TGF-β pathway data. Values are the mean ± SEM of a minimum of 3 independent experiments. *P<0.05; **P<0.005; ***P<0.0005; ****P<0.0001.