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. Author manuscript; available in PMC: 2022 Jul 12.
Published in final edited form as: Biochem Biophys Res Commun. 2021 May 27;562:133–138. doi: 10.1016/j.bbrc.2021.05.057

Figure 2. C. trachomatis affects the Hippo pathway in a Tarp-dependent manner.

Figure 2.

(A) HeLa cells were infected with either wild-type C. trachomatis L2 (WT), or a tarP deletion mutant (Δtarp) for 0-, 1-, 2-, 4-, 6- or 8-hours. Culture material was harvested, and protein lysates were generated. Proteins were resolved by SDS-PAGE and immunoblots were performed with actin (α actin), Tarp (α Tarp), large tumor suppressor (α LATS), Yes-associated protein (YAP) and WW domain-containing transcription regulator protein1 (WWTR1 also known as TAZ) are both recognized by the same polyclonal sera (α YAP/TAZ), mammalian sterile-20-like 1 (α MST1), mammalian sterile-20-like 2 (α MST2), phosphorylated YAP (α pYAPS397 and α pYAPS127), mps one binder kinase activator-like 1A and 1B (α MOB) and phosphorylated MOB (α pMOBT35) (B) Similar to (A) with the addition of uninfected control cells (uninfected) and HeLa cells infected with C. trachomatis mutant complemented with pTarp (Δtarp + pTarp) at 4-, 6- and 8- hours. Antisera specific for C. trachomatis heat shock protein 60 (α hsp60) served as an additional control.