Exosomes (EXO) were collected, and purified from A) Mouse HSCs isolated from normal liver after 1 (d1 mHSC) or 14 (d14 mHSC) days in culture; B) Rat HSCs isolated from normal liver after 1 (d1 rHSC) or 14 (d14 rHSC) days in culture; C) Human HSCs (hHSC) isolated from cultured TWNT4 cells. EXOs were analyzed for miR19b, 141, 145, 150 and 200 expression. *P<0.05 d14 versus d1 HSC-EXOs, n=3 independent experiments.