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Ectopic cartilage formation in L35 mut embryos, but not in A11 mut embryos. A-B: Frontal sections at eye levels were made and stained with safranin O (red) and counterstained with Fast Green (blue) at E15.5 or E17.5. Formation of massive ectopic cartilage is visualized in L35 mut embryos. Bar = 500 μm. C: Whole mount alcian blue staining at E17.5. Arrowheads, Meckel’s cartilage, ec, ectopic cartilage, t, tongue.
