Fig. 3.

Full-length SMAD6 rescues Notch loss-induced endothelial cell homeostatic flow mis-alignment. a Representative panels of HUVEC stained with Phalloidin (F-actin, white) with indicated siRNA treatments and SMAD6 expression construct under flow conditions. White arrow, flow vector. Red arrowheads, positive EC. Scale bar, 50 μM. b Quantification of cell axis ratio. Statistical analysis, One-way ANOVA; **p ≤ 0.01; ***p ≤ 0.001; ns not significant. N = 3, representative experiment shown. c Representative panels of HUVEC stained with Phalloidin (F-actin, red) with Notch1 siRNA treatments and expression constructs (full-length SMAD6, N-terminal SMAD6, or C-terminal SMAD6; green) under flow conditions. White arrow, flow vector. Green arrowhead, positive EC. Scale bar, 50 μM. d Quantification of cell axis ratio. Statistical analysis, One-way ANOVA; ****p ≤ 0.0001. N = 3, representative experiment shown. e Representative panels of HUVEC stained with Phalloidin (F-actin, red) with RPBJ siRNA treatment and expression constructs (full-length SMAD6, N-terminal SMAD6, or C-terminal SMAD6; green) under flow conditions. White arrow, flow vector. Green arrowhead, positive EC. Scale bar, 50 μM. f Quantification of cell axis ratio. Statistical analysis, One-way ANOVA; ****p ≤ 0.0001. N = 3, representative experiment shown