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. 2021 Jun 15;7:147. doi: 10.1038/s41420-021-00525-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A After PMA stimulated-differentiation, round and floating THP-1 cells became adherent flattened cells. B Flow cytometry analysis for the percentage of M2 polarized macrophages (CD206) after exposing to normoxia or hypoxia and co-culturing with or without 786-0 cells. C The RT–PCR detection of typical M2 markers (CD206, Arg1, IL-10, iNOS) in macrophages after exposing to normoxia or hypoxia and co-culturing with or without 786-0 cells. D Representative TEM images of TAM-derived exosomes (TAM-exo). Scale bar = 100 nm. E Size distributions of exosome fractions isolated from TAM conditioned medium by nanoparticle tracking analysis. F Western blot analysis of the exosomal markers (CD81, CD9, and CD63). *P < 0.01 vs. normoxia group.