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. 2021 Jun 15;11:11362. doi: 10.1038/s41598-021-90598-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Taxonomic eDNA detection curves. Relationship between the eDNA detection probability at a site (y-axis, right side), the average camera trap detection rate at the catchment (x-axis), and the rain the day prior to sampling (no rain in red, 30 mm in blue). Curves are calculated for 60 L of water filtered from a stream draining a 500 km² catchment. The figure shows the mean predicted value (solid lines) and associated standard errors (± SE, grey areas), calculated using the parameter estimates from the model with the lowest AICc (Supplementary Tables 2a,b). The histograms represent the observed density distribution of the average camera trap detection rates associated with eDNA sampling sites (y-axis, left side). For instance, on the “Ground rodents”, the histogram indicates that more than 75% of the eDNA sampling locations were associated with a catchment where on average across all camera trap located in the catchment, less than 0.2 ground rodents were detected every day, or one ground rodent detected every 5 days. Data from 2018 and 2019 are combined.