Figure 3.

PLNC8 αβ reduces inflammatory responses of S.aureus-infected human keratinocytes. (A) Representative images of HaCaT cells infected with S. aureus for 1 h, followed by addition of PLNC8 αβ for 24 h, scale bar is 50 µm. (B) PLNC8 αβ alone is not cytotoxic, and higher concentrations of the peptide substantially prevented S. aureus-mediated cytotoxicity. The increased secretion of (C) IL-6 and (D) CXCL8 by S. aureus was significantly reduced by the peptides. ***p < 0.001 (One-way ANOVA with Sidak’s post-hoc test).