Figure 3.

GRm L773P exhibits altered DNA binding properties. (A) Sedimentation velocity AUC experiment showing that both GRmL773P and GRm are competent in DNA binding. A slightly reduced s-value can be observed for GRmL773P + DNA. (B) EMSA assay under native conditions shows a high population of monomeric GRmL773P bound to DNA that is hardly visible for GRm, which forms dimers in the presence of DNA. Shown is a fluorescence scan that specifically detects DNA-containing bands and below a Coomasie stain to also detect protein species. Non-cropped gel images are presented in Supplementary Figure S6.