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. 2021 Jun 15;11:12515. doi: 10.1038/s41598-021-92039-9

Figure 6.

Figure 6

Single point mutation L773P alters the dynamics of several GR elements in the presence of Hsp90β. (A) Projection of the differential H/DX data for GRm and GRm L773P in the presence of Hsp90β onto the crystal structure of the GR DBD in complex with an fkbp5-derived GRE (PDB ID 3G6R). Significant changes are color coded in blue (low exchange) to red (high exchange). (B) Uptake plots for GR residues 444–461 that consist the DNA-reading helix in the absence and presence of Hsp90β. (C,D) Wood’s plots based on two experiments constructed with the Deuteros software for time points of 30 and 120 min using hybrid statistics (p-value < 0.01) to compare GRm and GRm L773P in the presence of Hsp90β and ATP. Persisting differences in the presence of Hsp90β concern the H1, H3, H4/5 and H7. (E,F) The most significant relative changes in the fractional uptake between the two GR variants that persist in the presence of Hsp90β and ATP are mapped on the GR LBD structure for 30 min and 2 h of exchange and are color coded in blue (low exchange) to red (high exchange).