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. 2021 Jun 15;12(6):617. doi: 10.1038/s41419-021-03891-6

Fig. 7. METTL14 promotes PTC cell proliferation, migration/invasion, and activate downstream EGFR, Akt, and MEK/ERK pathways through lncRNA OIP5-AS1.

Fig. 7

A, B MTT and colony-forming growth assays were performed to determine the proliferation ability of K1 and TPC-1 cells (n = 3). C Transwell assays were performed to determine the migration and invasion capacity of K1 and TPC-1 cells. Scale bars = 50 μm. The migration and invasion abilities (fold change of migrated or invaded) were calculated and compared to the different vectors in K1 and TPC-1 cells (n = 3). D Wound healing assays were performed to assess the migratory capacity of K1 and TPC-1 cells. The migratory activity (wound healing) was calculated and compared to vectors at 0 h (n = 3). E Western blot analysis of phosphorylated EGFR and total EGFR in TPC-1 and K1 cells (n = 3). F Phosphorylated and total Akt, ERK, and MEK levels measured by western blot analysis in TPC-1 and K1 cells (n = 3). *p < 0.05 and **p < 0.01.