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. 2021 Jun 15;12(6):614. doi: 10.1038/s41419-021-03876-5

Fig. 6. miR-21-5p interactes with MARCH5 and regulates its expression.

Fig. 6

A Binding sites of miR-21-5p and MARCH5 using Starbase 3.0. B Relative luciferase activity from reporter assays in cardiomyocytes co-transfected with mimic-NC/miR-21-5p mimic or inhibitor-NC/miR-21-5p inhibitors along with MARCH5-wt or MARCH5-mut (n = 3). C, D The mimic-NC and miR-21-5p mimic were intraperitoneally administered 24 h before myocardial I/R injury and infarction size (%) and levels of miR-21-5p expression were evaluated (n = 6). E Cardiomyocytes were transfected with mimic-NC and miR-21-5p mimic, followed by induction of H/R. Expression of miR-21-5p was evaluated by qRT-PCR (n = 3). F, G Cardiomyocytes were co-transfected with mimic-NC/miR-21-5p mimic and Vector/MARCH5 or inhibitor-NC/miR-21-5p inhibitor and sh-NC/sh-MARCH5 followed by induction of H/R. MARCH5 expression was evaluated by western blot and qRT-PCR (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001 and n.s no significance.