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. 2021 Jun 15;12:3644. doi: 10.1038/s41467-021-23902-6

Fig. 2. BTP or CMH has similar effects as TBH does.

Fig. 2

a Variant compounds having similar functional group with TBH. b U2OS control, ACSL4−/−; GPX4−/− cells pre-incubated with Nutlin (10 μM) for 12 h were treated with BTP (300 μM), Nutlin (10 μM), and Ferr-1 (2 μM) as indicated for 8 h. BTP, butanone peroxide. c U2OS control, ACSL4−/−; GPX4−/− cells pre-incubated with Nutlin (10 μM) for 12 h were treated with CMH (200 μM), Nutlin (10 μM), and Ferr-1 (2 μM) as indicated for 8 h. CMH, cumene hydroperoxide. d Lipid peroxidation levels of U2OS control, ACSL4−/−; GPX4−/− cells pre-incubated with Nutlin (10 μM) for 12 h were treated with TBH (300 μM), Nutlin (10 μM), and Ferr-1 (2 μM) as indicated for 8 h. e Quantification of lipid peroxidation levels is shown as in (d). b, c, e Error bars are mean ± s.d., n = 3 independent repeats. e All p values were calculated using two-tailed unpaired Student’s t-test. Detailed statistical tests are described in the ‘Methods’. For WT, p = 0.000000227, TBH versus Nutlin + TBH; p = 0.0000000407, Nutlin versus Nutlin + TBH. For ACSL4−/−; GPX4−/−, p = 0.000000000903, TBH versus Nutlin + TBH; p = 0.00000125, Nutlin versus Nutlin + TBH. Source data are provided as a Source Data file.