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Phosphorylation of contractile proteins. Phosphorylation levels of MyBP-C, desmin, cTnT, tropomyosin (Tm), cTnI, and MLC2 were significantly suppressed (A,C) in minipigs with HFpEF induced by chronic pressure overload. (A) Pro-Q phosphorylation staining image, each band represents an individual animal. n = 4 minipigs per group. *P < 0.05, **P < 0.01 vs. the sham group.
