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. 2021 Jun 15;11:12613. doi: 10.1038/s41598-021-91998-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Astrogliosis in ipsilateral thalamus is long-lasting and can eventually develop glial scar-like characteristics. Young mice were subjected to dMCAO and then allowed to recover for 6 weeks or two years. At 6 weeks or two years post-stroke, brains were evaluated for astrogliosis (GFAP). Brain sections showing astrogliosis at 6 weeks (A) or two years following stroke (B) (10X, stitched image; scale bar = 1 mm). (C) Enlarged region of thalamus from two year post-stroke brain showing glial scar-like characteristics (40X obj; scale bar = 50 µm). Two regions (1 and 2) were further enlarged to show different regions of gliosis.