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. 2021 Jun 15;12:3646. doi: 10.1038/s41467-021-23934-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a HeLa cells co-transfected with TSSC4-DDX6-dsRed fusion protein (red) and U5-specific proteins tagged with GFP or FLAG (turquoise). The DDX6/p54 peptide targets the TSSC4-DDX6-dsRed protein into cytoplasmic P-bodies. Co-recruitment of individual U5 proteins (PRPF8, SNRNP200, and EFTUD2) into P-bodies indicates their interaction with TSSC4. P-bodies marked by arrows are enlarged three times in insets. Scale bar represents 10 μm. b Quantification of P-body localization. The signal of GFP/FLAG-tagged U5-specific proteins in P-bodies was corrected by subtraction of the signal in the surrounding cytoplasm and compared to the signal in the nucleus. In total, 50 P-bodies were analyzed for each condition and shown in a box blot (center line—median; box limits—1st and 3rd quartiles; whiskers—5th and 95th percentile), two lowest and two highest values are depicted as separate dots. Statistical significance was determined by the two-tailed T test, and all P values lower than 0.0001 are indicated by ****. Original values for box plots are provided in the Source Data file.