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. 2021 Jun 15;12:3646. doi: 10.1038/s41467-021-23934-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Knockdown of TSSC4 by RNAi increased the accumulation of U4, U5, and U6 snRNAs in Cajal bodies. snRNAs detected by in situ hybridization (turquoise), Cajal bodies visualized by immunodetection of their marker coilin (red). Cajal bodies marked by arrows are enlarged 3 times in insets. Scale bars represent 10 μm. b Quantification of Cajal body accumulation. The fluorescence signal of in situ hybridization probes in Cajal bodies was normalized to signal in the nucleoplasm and shown in a box plot (center line—median; box limits—1st and 3rd quartiles; whiskers—5th and 95th percentile or 2.5th and 97.5th percentile for U2, U4 snRNAs, or U5, U6 snRNAs, respectively). Two lowest and two highest values are depicted as separate dots in each box plot. The number of analyzed cells is shown at the bottom of the diagram. Data for U2 and U4 snRNAs were measured in one experiment, data for U5 and U6 snRNAs were obtained in two biologically independent experiments. Statistical significance was determined by the two-tailed T test, and all P values lower than 0.0001 are indicated by ****. Original values for box plots are provided in the Source Data file.