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. 2021 Jun 15;12:3646. doi: 10.1038/s41467-021-23934-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Knockdown of TSSC4 by RNAi increased the accumulation of U5-specific proteins in Cajal bodies. PRPF19 was visualized by indirect immunofluorescence, the other proteins were tagged with GFP and stably (PRPF8-GFP and SNRNP200-GFP) or transiently (CD2BP2-GFP and EFTUD2-GFP) expressed in HeLa cells (turquoise). Cajal bodies were visualized by immunodetection of coilin (red). Cajal bodies marked by arrows are enlarged three times in insets. Scale bars represent 10 μm. b Quantification of Cajal body accumulation. Fluorescence signal in Cajal bodies was normalized to signal in the nucleoplasm and shown in a box plot (center line—median; box limits—1st and 3rd quartiles; whiskers—5th and 95th percentile). Two lowest and two highest values are depicted as separate dots in each box plot. The number of analyzed cells is shown at the bottom of the diagram. Statistical significance was determined by the two-tailed T test, and all P values lower than 0.0001 are indicated by ****. Original values for box plots are provided in the Source Data file. a, b Two biologically independent experiments were performed for each condition and combined.