Table 3.

Amplification conditions for the different groups of targeted organisms.

Targeted group of organisms	Amplification conditions
AM fungi (Glomeromycotina)	95 °C-3 min, 55 cycles, 95 °C-10 s, 60 °C-15 s, 72 °C-25 s
Eubacteria	95 °C-3 min, 55 cycles, 95 °C-10 s, 55 °C-20 s, 72 °C-25 s
Eukaryota, including protists	95 °C-3 min, 55 cycles, 95 °C-10 s, 48 °C-40 s, 72 °C-60 s
Internal DNA standard	95 °C-5 min, 50 cycles, 95 °C-10 s, 50 °C-20 s, 72 °C-10 s