Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 15;12:3648. doi: 10.1038/s41467-021-23951-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a–c The input VISTA image (a), the ground truth fluorescence image of lectin-DyLight594 stained blood vessels (b), and the predicted VISTA-lectin (v-lectin) image of blood vessels (c). d–f The input VISTA image (d), the ground truth fluorescence image of DAPI stained nuclei (e), and the predicted VISTA-DAPI (v-DAPI) image of nuclei (f). g–i The input VISTA image (g), the ground truth immunofluorescence image of MAP2 stained neuronal cell bodies and dendrites (h), and the predicted VISTA-MAP2 (v-MAP2) image of neuronal cells and dendrites (i). j Four-color volume imaging from label-free VISTA prediction for vessels (v-lectin, red), nuclei (v-DAPI, cyan), neuronal cell bodies, and dendrites (v-MAP2, green), and matured neuron cell bodies (v-NeuN, yellow). k Tandem 6-color volume imaging from label-free 4-color VISTA prediction and parallel two-color immuno-fluorescence images of GFAP (blue) and MBP (magenta). Scale bars: 40 μm. The length scale is in terms of distance after sample expansion.