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. 2021 Jun 15;11:12561. doi: 10.1038/s41598-021-92008-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fatty acid-suppressed steroidogenesis through impaired steroidogenic signaling is largely recovered by P5 treatment in MA-10 cells. (a) Representative blots in FA-induced MA-10 cells after 4-h 8-Br-cAMP treatment are shown. (b), (c), (d) Quantifications of blots normalized by β-actin or GAPDH are shown as means ± SEM (n = 3). (e) The progesterone levels from FA-induced MA-10 cells treated with or without 50 µM 8-Br-cAMP, 1 µg/mL of P5 and 10 µg/mL of 22R-OHC for 4 additional hours. The data are represented as means ± SEM (n = 3). *P < 0.05. n.s.: no significant difference.