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. 2021 Jun 15;11:12561. doi: 10.1038/s41598-021-92008-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rapamycin partially restores FA-suppressed basal steroidogenesis of MA-10 cells. After treatment with FA for 48 h and co-treatment with 10 nM rapamycin in the last 24 h, MA-10 cells were treated with or without 50 µM 8-Br-cAMP for 4 additional hours to induce steroidogenesis. (a) Progesterone levels in conditioned medium from the assigned groups of MA-10 cells. (b) Representative blots of autophagy and ER stress markers in cultured MA-10 cells are shown. (c), (d), (e), (f), (g), (h) Quantifications normalized by β-actin are shown as means ± SEM (n = 3). *P < 0.05.