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. 2021 Jun 15;11:12561. doi: 10.1038/s41598-021-92008-2

Figure 7.

Figure 7

Rubicon plays an indispensable role in maintaining and regulating the steroidogenesis of MA-10 cells, independent of the inhibition of late-stage autophagy. To further check the effects of late-stage autophagy inhibition on FA-suppressed steroidogenesis, we applied siRNAs to knock down Rubicon. After transfection with or without 10 nM Rubcn siRNAs for 72 h and treatment with 1% BSA or 1200 µM fatty acids (0.6 mM PA mixed with 0.6 mM OA) in the last 48 h, the conditioned medium and cell lysates from cultured MA-10 cells were collected for further analysis. (a) Progesterone levels in condition medium from the assigned groups of MA-10 cells. (b) Representative blots of autophagy and steroidogenesis markers in cultured MA-10 cells. (c), (d), (e) Quantifications normalized by GAPDH are shown as the means ± SEM (n = 3). *P < 0.05.