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On-chip generation of different H2O2 concentrations with a three-inlet design. Inlet 1 and 3 are loaded with HRP and amplex red, respectively, while inlet 2 isloaded with the mixture of H2O2 and amplex red. The flow rate ratio of the three inlets was set as (a) R1:R2:R3=5:1:24, (b) R1:R2:R3=5:15:10, (c) R1:R2:R3=5:20:5. R1, R2, R3 represent the flow rate from inlet 1, 2, 3 respectively.
