Figure 2.

Response of Mrgprd^Cre lineage afferents to optogenetic stimulation of the skin. (A) Schematic of cutaneous stimulation and intracellular DRG recordings using the ex vivo preparation. (B) Representative responses of Mrgprd^Cre lineage afferents to cooling, heating, mechanical, and optogenetic stimulation. (C) Representative responses to optogenetic stimulation of Mrgprd^Cre lineage afferents of varying duration and frequency. (D) Proportion of C fibers that respond to optogenetic stimulation of the skin in Mrgprd^Cre; Ai32 and Trpv1^Cre; Ai32 mice, as indicated. (E) Representative recording and quantification of the number of action potentials observed in a given cell on optogenetic stimulation of the skin in Mrgprd^Cre; Ai32 and Trpv1^Cre; Ai32 mice, as indicated. Data are mean ± SEM with dots representing data from individual neurons; n = 8 and 5 neurons, respectively, from at least 3 mice. N.S. indicates P > 0.05 (unpaired Student t test). DRG, dorsal root ganglia.