Figure 5.

Lamina I neurons receive strong input from Trpv1^Cre lineage afferents but weak input from Mrgprd^Cre lineage afferents (A) Schematic of recordings with optogenetic stimulation at either the peripheral or central terminal. (B–C) Representative traces and quantification of EPSC amplitude from lamina I neurons after optogenetic stimulation at either the skin or the spinal cord. *P < 0.05 (paired Student t test). (D and E) Representative voltage clamp recordings (V_H = −70) and quantification of the proportion of lamina I neurons that show EPSCs on optogenetic stimulation at the spinal cord in Mrgprd^Cre; Ai32 and Trpv1^Cre; Ai32 mice. (F–G) Representative current clamp recordings and quantification of the proportion of lamina I neurons that show action potentials on optogenetic stimulation at the spinal cord in Mrgprd^Cre; Ai32 and Trpv1^Cre; Ai32 mice. For D and F, the responses to 10 stimulus presentations are superimposed. EPSC, excitatory postsynaptic currents.