FIGURE 7.

Morphology of HAT-7 spheroids cultured in Matrigel and Hepato-STIM medium in the absence (control) or presence of fluoride (0.1–3.0 mM). (A) Spheroid images obtained on days 1, 2, and 7 when fluoride (0, 0.1, 0.3, 1.0, 3.0 mM) was added to the culture medium 24 h after seeding. Also shown are spheroids isolated from the Matrigel on day 7. (B) Spheroid images obtained on days 4, 5, and 7 when fluoride (0, 0.1, 0.3, 1.0, 3.0 mM) was added to the culture medium at the time of rapid spheroid growth 4 days after seeding. Also shown are spheroids isolated from the Matrigel on day 7. Scale bars: 70 µm.