Figure 1.

Lymphoid development in Tg^MOG mice. (A) Flow cytometry analysis of thymus from Tg^MOG and WT non-transgenic littermates. Representative plots are shown (top). Frequencies and total numbers of CD4⁺, CD8⁺, DN (CD4^–CD8^–), and DP (CD4⁺CD8⁺) T cells are depicted (bottom). (B) Cellularity of thymus, spleen, lymph nodes (LN), and bone marrow (BM). (C) Immune cell composition of the spleen from Tg^MOG and WT mice. Frequencies of CD19⁺, CD4⁺, CD8⁺, CD11c⁺, CD11b⁺, Gr-1⁺ and NK1.1⁺ cells in the live gate are shown. (D) B cell subsets in the spleen of Tg^MOG and WT mice. Frequencies of CD86^+,CD21^hiCD23^–, CD21^lowCD23^–, CD21^medCD23⁺ cells in gated B220⁺ cells are shown. (E) Activation marker expression in splenic CD4⁺ T cells. Frequencies of CD62L^low, CD69⁺, PD-1⁺, CD25⁺, Foxp3⁺ cells (left) or mean fluorescence intensity (MFI) of CD44 expression (right) in gated CD4⁺ cells are shown. (F) Representative flow cytometry histograms showing membrane MOG expression in lymphoid organs. (A–E) The combined data from two independent experiments is shown. n = 4 - 6 mice each group. Each circle represents an individual mouse.