Figure 3.

MOG-specific B cells are deleted in IgH^MOG X Tg^MOG mice. (A), Representative flow cytometry data from Tg^MOG, IgH^MOG, IgH^MOG X Tg^MOG mice. Cells were stained with antibodies against transgenic anti-IgM^a, WT anti-IgM^b, anti-MOG 8.18C5 antibodies or recombinant MOG protein along with B cell lineage markers B220 and CD19. Representative data from two independent experiments is shown. (B, C), MOG-specific (B), and total (C) IgM^a and IgG1^a antibodies in the sera were quantified by ELISA. Each circle represents an individual mouse. n = 5 – 8 mice per group. **P = 0.0016 (Mann-Whitney’s U-test, two-tailed).