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. 2021 Jun 16;521:9–18. doi: 10.1016/j.cca.2021.06.016

Fig. 5.

Fig. 5

Linearity assessment of RNA quantification. A: LOAA results showing amplification curves for each partition over cycles (upper) and scatter plots (lower). Red bars indicate the set threshold. B: Representative QX200 results showing an end-point one-dimensional amplitude plot. The pink line indicates the set threshold. C: Log-scale graph showing the linear correlation between the input SARS-CoV-2 RNA amount and the copy number concentrations from QX200 (red) and LOAA (blue). D: LOD and LOQ values for SARS-COV-2 quantification using the QX200 and LOAA (RdRP-1 assay). E: Log-scale graph showing the linear correlation between input HIV-1 RNA amount and copy number concentrations from QX200 (red) and LOAA (blue) using A assay. F: LOD and LOQ values for HIV-1 quantification using the QX200 and LOAA (A assay). Error bars indicate the SD with the mean of replicated measurements (n > 10 per sample).