Table 1.
Sequences of the hydrolysis probes and primers for the QX200 and LOAA
| Target | Probe sequences | Forward/Reverse Primers | |
|---|---|---|---|
| EGFR | T790M | MT 5′-[6-FAM]-CATCATGCAGCTCATGCCC-[BHQ1]- 3′ | 5′-CATCTGCCTCACCTCCAC-3′ |
| MT 5′-[6-FAM]-CATCATGCAGCTCATGCCC-[SFCQ]- 3′ | 5′- ACCAGTTGAGCAGGTACTGG-3′ | ||
| WT 5′-[HEX]-CATCACGCAGCTCATGCC-[SFCQ]- 3′ | |||
| WT 5′-[SFC620]-AAA-[FAM]-CATCACGCAGCTCATGCC-[SFCQ]- 3′ | |||
| L858R | MT 5′-[6-FAM]-GATTTTGGGCGGGCCAAACT-[SFCQ]- 3′ | 5′- CCAGGAACGTACTGGTGAAA-3′ | |
| WT 5′-[HEX]-GATTTTGGGCTGGCCAAACT-[SFCQ]-3′ | 5′- TACTTTGCCTCCTTCTGCAT-3′ | ||
| WT 5′-[SFC620]-AAA-[FAM]-GATTTTGGGCTGGCCAAACT-[SFCQ]-3′ | |||
| SARS-CoV-2 | RdRP-1* | 5′-[6-FAM] - CAGGTGGAACCTCATCAGGAGATGC - [BHQ1]-3′ | 5′-GTGARATGGTCATGTGTGGCGG-3′ |
| 5′-CARATGTTAAASACACTATTAGCATA-3′ | |||
| RdRP-2 | 5′-[6-FAM]-CCGTAGCTGGTGTCTCTATCTGT-[SFCQ]-3′ | 5′-TGCAAAGAATAGAGCTCGCA-3′ | |
| 5′-CTCCTCTAGTGGCGGCTATT-3′ | |||
| E | 5′-[6-FAM]-TCTTGCTTTCGTGGTATTCTTGCT-[SFCQ]-3′ | 5′-CGGAAGAGACAGGTACGTTAA-3′ | |
| 5′-GCAGTAAGGATGGCTAGTGT-3′ | |||
| N | 5′-[6-FAM]-CACCAATAGCAGTCCAGATGACC-[SFCQ]-3′ | 5′-ACTCAACATGGCAAGGAAGA-3′ | |
| 5′-GCTCTTCGGTAGTAGCCAAT-3′ | |||
| HIV-1 | A assay | 5′-[6-FAM]-CCAGGGATGGATGGCCCAAAAGTTAAACAATG-[BHQ1]-3′ | 5′- CCAATWAGTCCTATTGAAACTGTKCCAGT-3′ |
| 5′-RYTAATGCTTTTATTTTYTCTTCTGTCAATG-3′ | |||
| B assay | 5′-[6-FAM]- AGAT/ZENTM/CTGGCCTTCCTACAAGGGAAG-[IBFQ]-3′ | 5′- ATGGGTGCGAGAGCGTCA-3′ | |
| 5′-GTTGGCTCTGGTCTGCTCTG-3′ | |||
FAM, fluorescein; HEX, hexachloro-fluorescein; MT, mutant allele; WT, wild-type allele; *, assay from Charité Virology, Germany [14].