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Placental angiogenic balance is disrupted in GDM. Placental slides from women with GDM versus age, BMI and foetal sex matched controls were stained with (A) FKBPL, (B) SIRT-1, (C) PlGF and (D) VEGF-R1 primary antibodies, followed by staining with green Alexaflour or red Cy3 secondary antibody. Six images per slide were taken at 20x magnification and the mean fluorescence quantified using Image J. Representative images inset. SIRT-1: Mann-Whitney test; n=6, *<0.05.
