Figure 4.

FKBPL and SIRT-1 protein expression is reduced in ACH-3P cells in high glucose conditions in the presence of low oxygen levels. ACH-3P cells were exposed to high glucose HG, (25mM) in the presence of varying oxygen (O₂) conditions for 24 h before protein was extracted and Western Blotting performed. (A) FKBPL and SIRT-1 protein expression is reduced by low oxygen (2.5%) conditions at normal glucose concentration. (B) FKBPL and SIRT-1 protein expression remained unchanged in normal experimental oxygen conditions in the presence of high glucose compared to normal glucose. (C) FKBPL and (D) SIRT-1 protein expression was reduced in high glucose conditions at 6.5% and 2.5% O₂ compared to normal glucose conditions at the same oxygen levels. Protein expression was normalised to GAPDH. (n=3, (A) one-way ANOVA with Bonferroni post-hoc test’ (B–D) unpaired t-test, *<0.05).