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. 2021 Jun 2;12:650328. doi: 10.3389/fendo.2021.650328

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Copyright © 2021 Alqudah, Eastwood, Jerotic, Todd, Hoch, McNally, Obradovic, Dugalic, Hunter, Holmes, McCance, Young, Watson, Robson, Desoye, Grieve and McClements

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Angiogenic potential is further reduced with FKBPL overexpression in high glucose conditions in HUVECs. The number of (A) junctions and (B) branches was quantified following FKBPL overexpression in HUVECs for 24 h. The cells were stained with calcein stain, before being plated in Matrigel and exposed to high glucose (HG, 30 mM) or normal glucose (NG, 5.5 mM) media for 24 h. Six images/well were taken using a DMi8 microscope; representative images are shown inset. Images were analysed based on the number of junctions and branches formed using ImageJ angiogenesis macros. (n=6, *<0.05, **<0.01, ***<0.001, One-way ANOVA, followed by Bonferroni multiple comparison test).