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. 2021 Jun 2;9:686886. doi: 10.3389/fcell.2021.686886

FIGURE 1.

FIGURE 1

Effect of PDGF-D on macrophage proliferation and migration. (A) Immunoblotting showing the expression of PDGFR-α, PDGFR-β and NRP1 in RAW264.7 mouse macrophages, THP1 human monocytes and dTHP1 human macrophages. (B) Proliferation of mouse and human macrophages treated with PDGF-D protein for 12 or 24 h. (C,D) Migration of mouse (C) and human (D) macrophages stimulated with PDGF-D protein for 24 h. (E) Proliferation of mouse and human macrophages treated with conditioned medium from PDGF-D-treated HRPE cells (PDGF-D-CM). Conditioned medium from HRPE cells without PDGF-D treatment (CTL-CM) was used as a control. (F,G) Migration of mouse (F) and human (G) macrophages treated with conditioned medium from PDGF-D-treated HRPE cells (PDGF-D-CM). Conditioned medium from HRPE cells without PDGF-D treatment (CTL-CM) was used as a control. Scale bars: 400 μm. All the experiments were performed in triplicates. Unpaired two-tailed Student’s t-test was used for statistical analysis. *p < 0.05, ***p < 0.001, ****p < 0.0001, ns: not significant.